ANALYZING THE GENETIC DIVERSITY OF KAKI PERSIMMON (DIOSPYROS LOTUS L.) BASED ON SSR AND ISSR DNA-MARKERS

Diospyros lotus L. plants growing in seven geographically remote cenopopulations in the Western Caucasus were studied for the first time. SSR and ISSR-markers were used to identify the genetic structure of D. lotus. 19 microsatellite (SSR) and 10 (ISSR) markers have been tested in total. It was found that out of 19 SSR markers, only three (ssrdk14, ssrdk26, ssrdk30) have revealed a clear polymorphism in populations of kaki persimmon. Out of 10 ISSR-markers, five (ISSR815, ISSR880, ISSR13, ISSR814.1, ISSR15) have showed high efficiency. The analysis of the population structure has showed three genetic clusters, of which the populations from v. Shkhafit and c. Gagra have formed two separate clusters, and all other populations have formed a mixed cluster. A low level of genetic diversity with an insignificant amount of genetic impurities has been established. This suggests that D. lotus was introduced to the Western Caucasus from a single source of germplasm. The results obtained are an important basis for the implementation of environmental measures.