SPECIFICS OF THE INFLUENCE OF CYTOKININ TYPES AND CONCENTRATIONS DURING MICROPROPAGATION STAGE OF THE GENUS ACTINIDIA LINDL. REPRESENTATIVES

Biotechnological methods of accelerated reproduction and preservation of valuable genotypes and creation of genetically diverse material for breeding may have a wide practical yield, only if stable mass production of regenerant plants is provided by induction of in vitro organogenesis. Due to the increased interest in nontraditional horticultural crop of kiwifruit and its successful breeding, there is a need for applying biotechnological methods of accelerated micropropagation. Species of the genus Actinidia Lindl. are garden crops whose fruits contain biologically active substances with antioxidant properties, including vitamins, catechins, pectins and many other compounds. Research object: shoots with apical and lateral buds of Actinidia deliciosa cultivars ‘Hayward’ and ‘Monty’ and A. arguta Planch. species, in active growth phase, 2.0–2.5 mm in size. The aim of the research was to study the influence of different cytokinin types and concentrations on micropropagation characteristics of the genus Actinidia Lindl. representatives. The stage of Actinidia deliciosa and A. arguta Planch micropropagation has been tried and tested. The optimum composition of growth regulators, increasing shoot multiplication factor, has been established for kiwifruit: A. deliciosa ‘Hayward’ (4.2 units/exl.) and ‘Monty’ (3.8 units/exl.) on 6 BAP (2 mg/l) and GA3 (1 mg/l) containing nutrient medium, for A. arguta (4.5 pcs/exl) on medium with kinetin at 1 mg/l and (3.6 pcs/exl) and on 6 BAP (2 mg/l) and GA3 (1 mg/l) containing medium (second passage). The influence of various factors on kiwifruit micropropagation rate has been found, the influence of genotypic features – 42 %, growth regulators – 30 %, and interaction of these factors – 23 %.